Significant difference in antibody response to epitopes of known melanoma antigens CSPG4 (#4), PMEL (#6) and TGO1 (#31) was noticeable between PEM-Mel and MelVac and their respective control groups (Wilcoxon Rank Sum test, p < 0.001, Fig. 3b, Supplementary Data 4). The gene discussed is PMEL; the disease is melanoma.