The result of differential analysis in normal breast tissue and tumor tissue showed that ACO1, CHMP6, ENPP2, MAFG, NEDD4L, PIR, SLC1A4, SLC7A5, and VEGFA had significant differential expression in breast cancer with p-value <0.001; ACADSB and NOX3 with p-value <0.01; and PEX12 with p-value <0.05 (Figure 1J). The gene discussed is ACO1; the disease is breast cancer.