Through the analysis of Kaplan–Meier plotter, GEPIA and HPA databases, it was found that high expression of PRKDC, FANCD2, POLD1 and RECQL4 genes is significantly correlated with shorter OS, while FANCD2 and POLD1 have not been reported to be associated with liver cancer, which may become biological markers to evaluate the prognosis of HCC patients and provide new ideas for the diagnosis and treatment of HCC. This evidence concerns the gene RECQL4 and hepatocellular carcinoma.