Thus, mRNA expression started already to increase after 15 min of induced middle cerebral artery occlusion peaking at 2 h after reperfusion in cortical neurons from the rat brain [40] and was accompanied by elevated levels of BDNF protein peaking at 2 h [72] of reperfusion (following 2 h of middle cerebral artery occlusion). The gene discussed is BDNF; the disease is medical procedure.