Studies have shown that interleukin-1β (IL-1β) and interleukin-18 (IL-18) are closely related to the production of islet beta cells and nitric oxide (NO) [14, 15]; so, we measured the levels of IL-18 and NO to investigate the effect of HIV-1 protease inhibitor on insulinoma. The gene discussed is IL18; the disease is pancreatic insulinoma.