The RNA expression data of the ZG16B gene (the PAUF coding gene) in cancer cell lines (Supplementary Figure S4), show that high levels of PAUF expression can be found in a much higher portion of breast cancer and gastrointestinal cancer (pancreas, bile duct, gastric, and colorectal) cells, while ovarian cancers express a relatively low level of PAUF on average, but a small portion (around 10%) of the ovarian cancer cells expressed high level of PAUF. This evidence concerns the gene ZG16B and breast cancer.