Indeed, even assuming 100% loading efficiency, the amount of tPA delivered via FSNs would be 1.45 μg/mg of FSNs with the above loading conditions, and at the 20 mg/kg tPA‐FSN dose examined here, 0.029 mg/kg tPA would be delivered, which is ~30 times less than the therapeutic dose for stroke (0.9 mg/kg) yet more than the endogenous tPA concentrations of 0.1 nM.27 This evidence concerns the gene PLAT and Stroke.