The luciferase assay showed that transfection with miR-195-5p mimics significantly reduced the relative luciferase activity of PTBP1-3′-UTR-wild-type-treated lung cancer cells; however, it did not affect that of PTBP1-3′-UTR-mutation-treated lung cancer cells (Figure 6E). This evidence concerns the gene PTBP1 and lung cancer.