In another example, a NSD1 knockout cell line was constructed by a CRISPR/Cas9 editing system to demonstrate its potential therapeutic application to HCC cell proliferation, migration and invasion as a result of NSD1 positive role in the expression of histone H3, Wnt10b and Wnt/β-catenin signaling (84). This evidence concerns the gene NSD1 and hepatocellular carcinoma.