GFAP and Other metabolic disease: Also, in agreement with our studies showing an overactivation of the CBs in metabolic disease states (Ribeiro et al., 2013; Dos Santos et al., 2018; Ribeiro et al., 2018; Cunha-Guimaraes et al., 2020) and with the work of Cramer that found that CBs from type 2 diabetic patients are 25% bigger than those of healthy individuals (Cramer et al., 2014); herein, we observed that short-term HFHSu diet intake significantly increases the number of TH-positive and GFAP-positive staining, suggesting that these increased number of cells might contribute to an increased function of this organ.