Compared with the control group, the expression levels of TLR4, NF-κB, and the proapoptotic protein Bax in the intestinal tissues of the NEC group were significantly increased (P < 0.01), and the expression of the antiapoptotic protein Bcl-2 was significantly decreased (P < 0.01). The gene discussed is NFKB1; the disease is necrotizing enterocolitis.