After EA intervention, the expression of NLRP3, pro-caspase-1, cleaved caspase-1 p20, pro-IL-1β, cleaved IL-1β, and GSDMD in C57 mice decreased significantly, and the expressions of NLRP3 in Cas-1 ko mice decreased significantly, indicating that EA could inhibit the expression of NLRP3, pro-caspase-1, cleaved caspase-1 p20, pro-IL-1β, cleaved IL-1β, and GSDMD in hippocampus after cerebral ischemia/reperfusion. This evidence concerns the gene NLRP3 and Cerebral ischemia.