It is important to note that in our previous study,21 while CD274 CN gains with at least ploidy +2 was positively correlated with PD-L1 IHC in NSCLC, there was a subset of PD-L1-positive patients that were negative for CD274 CN gain and a subset of PD-L1-negative patients that were positive for CD274 gain at a threshold of at least ploidy + 2, indicating that CD274 CN positivity could be an independent predictive biomarker of ICPI response. The gene discussed is CD274; the disease is non-small cell lung carcinoma.