To further analyse the suppressive immune milieu in highly immunogenic ICCs, we focused on activated CD8+ tumour-infiltrating lymphocytes (TILs) as effector cells, tumour-infiltrating regulatory T cells (Treg) as suppressor cells, TIM-3 as an immune checkpoint molecule and HLA-A, based on the immunophenoscore analysis. Here, HLA-A is linked to neoplasm.