The three CCA organoid lines express similar levels of checkpoint molecule B7-H3 (CD276) and CD155 (possible therapeutic immunological target), which was expressed on almost every cell (B7-H3: 94.2–99.9%, CD155: 94.3–98.1%), while major histocompatibility complex (MHC) class 2 molecules (2.6–5.0%), B7-H5 (0.5–1.4%), and co-stimulatory molecule CD80 (0.1–2.2%) were hardly present on CCA organoids (Fig. 5a, b and Supplementary Fig. S4). Here, PVR is linked to cholangiocarcinoma.