In this study, by employing hematoxylin and eosin (HE) staining, periodic acid-Schiff (PAS) staining, picrosirius red staining, and Masson staining, we noted kidney fibrosis, including glomerulosclerosis and tubulointerstitial fibrosis, in RTN3-knockout (RTN3-null) mice at 13 months of age. Here, RTN3 is linked to glomerulosclerosis.