Pathway analysis of RNA sequencing data from tumours from Stat1+/+ and Stat1−/− mice orthotopically injected with LY2 HNSCC cells also identified TRIM24 as the top predicted negative transcriptional regulator of STAT1 during HNSCC in our data set (z score –5.804, p value 1.32 × 10−16) (Fig. 5a, b). The gene discussed is TRIM24; the disease is head and neck squamous cell carcinoma.