Moreover, patients with ESCC presenting lymph node metastasis (N1–3) and a high American Joint Committee on Cancer (AJCC) seventh stage (stages III/IV) had higher WNT5A expression than those without lymph node metastasis (N0) and with a low seventh edition AJCC stage (stages I/II) (P < 0.001, Fig. 1B). This evidence concerns the gene WNT5A and esophageal squamous cell carcinoma.