WNT5A and esophageal squamous cell carcinoma: We subsequently examined the expression of HDACs after WNT5A overexpression or knockdown in ESCC cells using western blotting to explore the underlying molecular mechanisms by which WNT5A overexpression promotes ESCC metastasis and found that only the expression of HDAC7 was upregulated or downregulated, while other HDACs showed no changes (Fig. 4A and Supplementary Fig. S2A–D).