To investigate the role of HDAC7 in ESCC metastasis, we firstly assessed HDAC7 expression in multiple ESCC cell lines (Fig. 4B), and then we established two stable cell lines via lentiviral infection (HDAC7-overexpressing EC109 cell line and HDAC7 knockdown EC9706 cell line). This evidence concerns the gene HDAC7 and esophageal squamous cell carcinoma.