To explore Kindlin-2 deficiency-induced inhibition of AR signaling and breast cancer progression is due to the reduction of AR Tyr-534 phosphorylation, we used a phosphor-mimic mutant AR-Y534D (tyrosine 534 replaced by an aspartic acid) to study the function of AR Tyr-534 phosphorylation. This evidence concerns the gene FERMT2 and breast carcinoma.