Consistent with the in vitro findings (Figs. 1B and 2D), significant reduction of AR Tyr-534 phosphorylation and cyclin D1 expression was observed in the tumor sections (Fig. 5A, B) and isolated primary tumor cells (Fig. 5C) from the PyMT; Kindlin-2 cKO mice compared with those from the PyMT; WT mice, suggesting that loss of Kindlin-2 diminishes AR Tyr-534 phosphorylation and cyclin D1 expression. This evidence concerns the gene AR and neoplasm.