Four different groups P2X4/NR (293T stably expressing P2X4 protein and inoculated with control or normal human sera), NV/NR, (293T stably transfected with vector alone or null vector and inoculated with control or normal human sera), P2X4/HCV (293T stably expressing P2X4 protein and infected with human HCV sera) NV/HCV (293T stably transfected with vector alone or null vector and infected with human HCV sera) were established and successfully maintained till day 5 and day 9 post infection. This evidence concerns the gene P2RX4 and infection.