In addition, since gastric cancer stem cell (GCSC) is reportedly involved in both distant metastasis and metabolic reprograming of cancer cells[9], we also measured the mRNA expression levels of GCSC markers including CD44, EpCam and Lgr5 in both groups and found that all three markers were overexpressed in the ectopic CPT1C expression group compared to those in the empty vector-transduced group (Figure 2F). This evidence concerns the gene CPT1C and cancer.