To test this, we first aimed to exclude the influence of β1AR on NNK‐induced pancreatic cancer βAR activation, and the results showed that β1AR knockdown (Fig. 5A) had no effect on the activation of Akt (Fig. 5B); however, β2AR knockdown and inhibition significantly suppressed Akt activation (phosphorylation) and LC3 maturation (Fig. 5B,C), which suggested that the β2AR‐Akt axis was the main pancreatic cancer cells autophagy inducer after NNK stimulation. This evidence concerns the gene AKT1 and familial pancreatic carcinoma.