However, our study also had some limitations, the concrete carcinogenic mechanism of PITX2, PRKAA2, SFN, and CDK1 in HCC should be further explored in cell and animal experiments, and the expression levels of PITX2, SFN, CDK1, and PRKAA2 should be verified in larger size of clinical HCC samples. Here, PRKAA2 is linked to hepatocellular carcinoma.