Furthermore, western blot analysis indicated that phosphorylated ASK1, phosphorylated JNK and BAX expression were increased, whereas BCL-2 level was decreased in SRP9-knockdown cells compared with their counterparts, demonstrating that SRP9 regulated apoptosis also through the ASK1/JNK pathway in CRC cell lines (Fig. 5g). This evidence concerns the gene MAP3K5 and colorectal carcinoma.