In contrast, the few limited genome-wide sequencing-based studies of ALL subtypes have focused on specific subtypes, such as ETV6-RUNX1, high hyperdiploidy, unclassified B cell ALL (B-ALL) and T cell ALL (T-ALL) and reported contradicting results ranging from a mild increase in global DNA methylation28 to significant hypomethylation29,30. This evidence concerns the gene RUNX1 and acute lymphoblastic leukemia.