Other deleterious pos-pos variants found in MACF1 and ZBTB20-AS1 align with earlier studies showing that MACF1 mutations detected in tissue-specific genomes are responsible for function dysregulation associated with cancer67, and a correlation study found that key ZBTB20-AS1 lncRNAs are associated with colon tumor staging and likely tumor progression68. Here, PTGDR is linked to colonic neoplasm.