To gain a deeper understanding of the function of Prrx1 in CAFs, the effect of Prrx1 knockdown in representative mouse fibroblasts, such as MEF, MMTV-CAF (CAF derived from MMTV-PyMT mouse cancer), and wound-healing fibroblasts (fibroblast derived from mouse wound-healing tissue), were investigated. The gene discussed is PRRX1; the disease is cancer.