In addition, there were no statistically significant differences in the clinical parameters of histological differentiation grade, CA19-9 level, and tumor location among the three CRC groups (NM, LMN, and DM) in the TMT and PRM analysis; CEA level, CA19-9 level, and tumor location showed significant difference among the three CRC groups in the dot blot analysis. The gene discussed is CEACAM5; the disease is colorectal carcinoma.