To determine whether such differential effects are also evident when the two p53 mutants are expressed endogenously, we employed RNP-mediated CRISPR/Cas9 gene editing to replace the endogenous wild type TP53 genes of HCT116 CRC cells with either p53R273H or p53R175H (Fig. 3a). Here, TP53 is linked to colorectal carcinoma.