To further assess the generality of the R273 signature, we expressed p53R273H and p53R175H ectopically in two additional CRC-derived cell lines: RKO cells, depleted of their endogenous wtp53 (KO)34, and COLO-205 cells, which endogenously express truncated p53 (Supplementary Fig. 3b, d). This evidence concerns the gene TP53 and colorectal carcinoma.