To elucidate the role of Tubb4a in spontaneous prostate cancer models, we crossed Nkx3-1CreERT2 knock-in mice with Tubb4a floxed mice and/or Pten floxed mice to create prostate conditional Tubb4a single prostate conditional KO (cKO) (Tubb4a-cKO), Pten single cKO (Pten-cKO), and Tubb4a and Pten double cKOs (Tubb4a/Pten-cKO) mouse models on a C57BL/6 background (Figs. S11A–C). Here, PTEN is linked to Familial prostate cancer.