To further determine the effects of Cal/ICG@MPs-induced CAF regulation on the intratumoral CD8+ T cells, stroma-rich H22 tumor-bearing mice were intravenously injected with PBS, ICG, Cal/ICG, ICG@MPs, Cal@MPs or Cal/ICG@MPs twice every 2 days, followed with 808 nm laser irradiation at tumor sites for 10 min, and then the distribution of α-SMA+ CAFs and CD8+ T cells in tumor tissues was observed by immunofluorescence staining (Fig. 6f). The gene discussed is ACTA1; the disease is neoplasm.