To determine whether Cal/ICG@MPs-mediated PTT could induce effective ICD effects, H22 cells were treated with PBS, free ICG, Cal/ICG, ICG@MPs, Cal@MPs, or Cal/ICG@MPs in the presence or absence of 808 nm laser irradiation, and the hallmarks of ICD including surface calreticulin (CRT) expression and release of high mobility group box 1 (HMGB1) and ATP were then detected37–39. This evidence concerns the gene HMGB1 and impulse control disorder.