To further evaluate the antitumor activity of Cal/ICG@MPs upon 808 nm laser irradiation, the highly malignant bilateral 4T1 orthotopic tumor models were constructed by co-injection of 4T1 cells and TGF-β-activated skin fibroblasts into the left (the primary tumor) and right (contralateral tumor) breast fat pad on day 0 and 4, respectively. Here, TGFB1 is linked to neoplasm.