Because inhibitory neurons are commonly affected in neurodevelopmental disorders (Benes et al., 1998; Konradi et al., 2011; Schobel et al., 2013; Caletti et al., 2015; Reichel et al., 2015), we next asked whether HO produced long-term changes in the GAD65-expressing interneuron density of GAD65-GFP mice. This evidence concerns the gene GAD2 and neurodevelopmental disorder.