SCAF11 and infection: We then performed intranasal infection of C57BL/6 wild-type (WT) mice with pathogenic mouse-adapted SARS–CoV-2 (strain MA10) (20) and found that infection strongly induces Casp11 expression throughout murine lung tissue within 4 d of infection, as detected by RNAscope in situ hybridization (ISH) (Fig. 1D) and confirmed by qRT-PCR (Fig. 1E).