2021). Next, we sought to explore if quercetin reduces the expression levels of IL-6 and TNF-α. Quercetin with concentrations of 10, 20, 30 μM was applied to the cell of the CI-AKI model. Elisa assay was used to detect the expression levels of IL-6 and TNF-α. Results indicated that all three concentrations of quercetin significantly reduced the expression level of IL-6 and TNF-α in the CI-AKI model. Moreover, there was no difference in effect between quercetin of 20 and 30 μM (Figure 2D). The gene discussed is TNF; the disease is acute kidney injury.