The present study also confirmed that LLEFE containing these compounds inhibits the growth and proliferation of HT-29 colon cancer cells in vitro. LLEFE may effectively regulate the PI3K/Akt pathway, inhibit PI3K/Akt expression by the effective chemical compounds that it contains, control the level of oxidative stress, and inhibit colon cancer proliferation. Here, AKT1 is linked to colonic neoplasm.