When applied to mesenchymal and epithelial breast cancer cells, the analysis revealed that the splicing factors MBNL1, MBNL2 and RBFOX2 (RNA-binding fox-1 homolog 2) interact with autophagic components including RUBCN/Rubicon (rubicon autophagy regulator; a BECN1-interacting protein and inhibitor of PtdIns3K activity)39 and WDFY3/ALFY (WD repeat And FYVE domain containing 3; a scaffold protein for selective autophagy)40. This evidence concerns the gene RUBCN and breast carcinoma.