Re-examination of the kinetics of SmcHD1 association with Xi by immunofluorescence (IF) in our NPC model with inducible Xist expression confirms that SmcHD1 recruitment is a late step, becoming detectable only after 3–4 days of Xist induction and NPC differentiation (Figures 5A and 5B). This evidence concerns the gene SMCHD1 and nasopharyngeal carcinoma.