To determine transcriptional profiles, interrogate differentiation pathways, and examine antigen specificity of these PD-1-expressing cells, we performed scRNA-seq with TCR sequencing on sorted PD-1+ CD8+ T cells isolated from three non-small cell lung carcinoma (NSCLC) BrMs and two melanoma BrMs immediately after surgical resection (Figure S3). This evidence concerns the gene CD8A and melanoma.