To understand the potential effect of YTHDF2 on the TMZ resistance in GBM cells or the TMZ sensitivity of GBM resistant cells, LV‐YTHDF2 was infected into T98G and LN229 cells to enhance YTHDF2 expression, and LV‐sh‐YTHDF2 was infected into T98G/TR and LN229/TR cells to silence YTHDF2. This evidence concerns the gene YTHDF2 and glioblastoma.