The analysis of these cell cultures allows exploration of the unique genetic context of single tumors, thus revealing the presence of preexisting mutations in phosphatidylinositol-4,5-bisphosphate 3-kinase (PIK3CA) gene and the acquisition of new tumor-derived mutations in ESR1 gene, PIK3CA gene, and fibroblast growth factor receptor gene 2 (FGFR2), among others[19]. Here, PIK3CA is linked to neoplasm.