These cell lines, cultured for several months, showed up to have strong cancer stem cell phenotypical traits, with self-renewal and multilineage differentiation properties as well as metastatic potential and a robust expression of typical cancer stem cell markers, ranging from aldehyde dehydrogenase (ALDH1A1) and CD133 to CD26 and CD144V6[21]. This evidence concerns the gene PROM1 and cancer.