MGMT and central nervous system cancer: Figure 6B reveals representative dose-response profiles of TMZ, AFt-TMZ and AFt alone in U373M cells. As seen, AFt alone had no significant impact on cell growth or viability. It was also evident that, AFt-encapsulation of TMZ caused the MMR-deficient HCT 116 cell line to regain sensitivity to this alkylating agent (~25-fold reduction in GI50 value). Similarly encapsulation of TMZ in AFt cages restored sensitivity of SF188 paediatric glioma cells to TMZ. Resistance to TMZ is reported to be conferred by multiple mechanisms in SF 188 cells including PI3K signalling and MGMT expression[28,29].