Based upon a second review of immunohistochemical staining, all the stains showed aberrant nuclear localization of beta-catenin protein, which is an established indicator of activated CTNNB1 mutations (the gene that encodes the beta-catenin protein) [16, 21, 22] Previous studies have demonstrated that NMC and NMC-DTF both harbor identical CTNNB1 mutations, particularly CTNNB1 p. S45F, a specific CTNNB1 mutation that has been associated with more aggressive clinical behavior [21, 22]. Here, CTNNB1 is linked to nut midline carcinoma.