Several of the genes identified in the Prol cell-type have previously been associated with poor overall or recurrence-free survival outcomes in HCC, including PTMA [52, 68], HMGB2 [53], HMGB1 [54], H2AFZ [59], GAPDH [55], TUBB [57], STMN1 [56], and TUBA1B [58]. This evidence concerns the gene HMGB1 and hepatocellular carcinoma.