HSPA1A and amyotrophic lateral sclerosis: For Hsp70 prediction, TDP-43 was divided into three fragments: aa 1–120 (N-terminal domain and flexible linker of TDP-43 (NTD)), aa 101–269 (the two RNA recognition motifs (RRM)) and aa 270–414 (C-terminal prion-like domain, which is mostly unstructured, aggregation prone, and the site for most ALS mutations) (Fig. S2A).