To investigate the functional impacts of CUX2 variants appeared in patients with epilepsy (Fig. 1A, Table 1), we transfected HeLa.S3 cells with expression constructs of wild-type (WT) and the five variants (p.R34W, p.D337N, p.P454L, p.W958R, and p.E1283K). This evidence concerns the gene CUX2 and epilepsy.