To investigate whether CUX2 and its paralogues' mutations are involved in other types of epilepsies, here we performed targeted sequencing of CUX2, its paralog CUX1, and CASP which is a short isoform of CUX1 with a unique C-terminus, in Japanese patients with variable epilepsies including genetic generalized and structural/metabolic epilepsies, and identified their variants predominantly in patients with temporal lobe epilepsy (TLE), the most common but intractable form of epilepsy8. This evidence concerns the gene CUX1 and epilepsy.