The dose of I-125 for irradiation was chosen based on growth curve of PLC and Huh7 in normal control and I-125 incubation based on their IC50 in order to see the direct effect of I-125 on HCC proliferation, which shows the cell proliferation decreased as increased radiation dose (Figure 2(a)); the IC50 of PLC and Huh7 cells was 6.20 Gy and 5.39 Gy at 24 hours after irradiation, respectively. Here, HSPG2 is linked to hepatocellular carcinoma.