APC and neoplasm: Among the tumor suppressor genes, folic acid supplementation induced a significant change in promoter methylation of APC and P16. Promoter methylation was found to be significantly increased at APC promoters with the folic acid treatment increasing in JEG-3 cells by 1.14 fold (p < 0.05) and 1.28 fold (p < 0.01) at 10−7M and 10−4M folic acid treatment, respectively, compared to untreated control cells, while in HTR-8/SVneo cell APC methylation increased significantly by 4.1-fold at 10−7M only (Figure 5D).