While there is some inter-run variation, on average a large increase in MCT transporters (MCT1 and/or MCT4) is seen in CRC spheroids, with 1.438 fold difference in HCT116 in MCT4 and 1.827 (MCT1) and 1.280 (MCT4) fold difference in SW948. This evidence concerns the gene SLC16A1 and colorectal carcinoma.