HDGF and liver cancer: Therefore, liver cancer cell lines HLE, HLF, HepG2, and Huh6 were transiently transfected with siRNAs against HDGF. Successful knockdown of HDGF was verified by qRT-PCR (Fig. 5A, Additional file 1: Fig. S7A) and western blotting (Fig. 5B, Additional file 1: Fig. S7B).