The HCC cell line HepG2 carries a deletion of 116 amino acids in exons 3–4 in CTNNB1 (Fig. 5B) and Huh6 harbors an activating β-catenin mutation, both, resulting in constitutive activation of the Wnt/β-catenin signaling pathway [41, 42], whereas there is no detectable Wnt signaling in HLE and HLF cells [43, 44]. The gene discussed is CTNNB1; the disease is hepatocellular carcinoma.