RB1 and cancer: To this end, we conducted RNA-seq on cancer cell populations isolated by flow cytometry from Rb/p53/p130 triple knockout (TKO) tumors growing in the lungs of Rbfl/fl;p53fl/fl;p130fl/fl;Hes1GFP/+ mice following intra-tracheal instillation of an adenovirus expressing Cre (Ad-CMV-Cre).