To gain a broader and unbiased view of the phenotypes related to REST loss, we isolated NE and non-NE cells from TKO and TKO;Restfl/fl mutant mice (which were not crossed to the Hes1GFP allele) using the cell surface markers NCAM1 and ICAM1 based on the RNA-seq and FACS analysis of GFPhigh and GFPneg SCLC cells (Supplementary Data 1, Supplementary Fig. 6a,b and Fig. 3d). The gene discussed is REST; the disease is small cell lung carcinoma.